酶切突變工(gōng)具

輸入原始序列或1至多條FASTA格式序列，長度限定在10000以内。

>CDS1 atgtctgattcgctaaatcatccatcgagttctacggtgcatgcagatgatggattcgag ccaccaacatctccggaagacaacaacaaaaaaccgtctttagaacaaattaaacaggaa agagaagcgttgtttacggatctattcgcagatcgtcgacgaagcgctcgttctgtgatt gaagaagctttccaaaacgaactcatgagtgctgaaccagtccagccaaacgtgccgaat ccacattcgattcccattcgtttccgtcatcaaccagttgctggacctgctcatgatgtt ttcggagacgcggtgcattcaatttttcaaaaaataatgtccagaggagtgaacgcggat tatagtcattggatgtcatattggatcgcgttgggaatcgacaaaaaaacacaaatgaac tatcatatgaaaccgttttgcaaagatacttatgcaactgaaggctccttagaagcgaaa caaacatttactgataaaatcaggtcagctgttgaggaaattatctggaagtccgctgaa tattgtgatattcttagcgagaagtggacaggaattcatgtgtcggccgaccaactgaaa ggtcaaagaaataagcaagaagatcgttttgtggcttatccaaatggacaatacatgaat cgtggacagagtgacatttcacttcttgcggtgttcgatgggcatggcggacacgagtgc tctcaatatgcagctgctcatttctgggaagcatggtccgatgctcaacatcatcattca caagatatgaaacttgacgaactcctagaaaaggctctagaaacattggacgaaagaatg acagtcagaagtgttcgagaatcttggaaaggtggaaccactgctgtctgctgtgctgtt gatttgaacactaatcaaatcgcatttgcctggcttggagattcaccaggttacatcatg tcaaacttggagttccgcaaattcactactgaacactccccgtctgacccggaggaatgt cgacgagtcgaagaagtcggtggccagatttttgtgatcggtggtgagctccgtgtgaat ggagtactcaacctgacgcgagcactaggagacgtacctggaagaccaatgatatccaac aaacctgataccttactgaagacgatcgaacctgcggattatcttgttttgttggcctgt gacgggatttctgacgtcttcaacactagtgatttgtacaatttggttcaggcttttgtc aatgaatatgacgtagaagattatcacgaacttgcacgctacatttgcaatcaagcagtt tcagctggaagtgctgacaatgtgacagtagttataggtttcctccgtccaccagaagac gtttggcgtgtaatgaaaacagactcggatgatgaagagagcgagctcgaggaagaagat gacaatgaatag

* 目标酶切位點 AatI agg|cct AatII gacgt|c Acc16I tgc|gca AccII cg|cg AccIII t|ccgga AclI aa|cgtt AcvI cac|gtg AfaI gt|ac AfeI agc|gct AflII c|ttaag AgeI a|ccggt AhlI a|ctagt Alw441 g|tgcac AluI ag|ct Aor51HI agc|gct ApaI gggcc|c ApaLI g|tgcac AscI gg|cgcgcc AseI at|taat Asp718I g|gtacc AsuII tt|cgaa AvaI c|ycgrg AviII tgc|gca AvrII c|ctagg BalI tgg|cca BamHI g|gatcc BanIII at|cgat BbeI ggcgc|c BbrPI cac|gtg BbuI gcatg|c BcuI a|ctagt BclI t|gatca BfaI c|tag BfrI c|ttaag BfrBI atg|cat BglII a|gatct BlnI c|ctagg BseCI at|cgat BsePI g|cgcgc BseX3I c|ggccg BshTI a|ccggt Bsp1407I t|gtaca Bsp19I c|catgg BspDI at|cgat BspEI t|ccgga BsrGI t|gtaca BssHII g|cgcgc BstUI cg|cg ClaI at|cgat DraI ttt|aaa EagI c|ggccg EcoRI g|aattc EcoRV gat|atc EgeI ggc|gcc FseI ggccgg|cc FspI tgc|gca HaeIII gg|cc HincII gty|rac HindIII a|agctt HinfI g|antc HpaI gtt|aac HpaII c|cgg KasI g|gcgcc KpnI ggtac|c MboI |gatc MfeI c|aattg MluI a|cgcgt MscI tgg|cca MseI t|taa MspI c|cgg NaeI gcc|ggc NarI gg|cgcc NcoI c|catgg NdeI ca|tatg NdeII |gatc NgoMIV g|ccggc NheI g|ctagc NotI gc|ggccgc NruI tcg|cga NsiI atgca|t PacI ttaat|taa PciI a|catgt PhoI gg|cc PmeI gttt|aaac PmlI cac|gtg PsiI tta|taa PstI ctgca|g PvuI cgat|cg PvuII cag|ctg RsaI gt|ac SacI gagct|c SacII ccgc|gg SalI g|tcgac SbfI cctgca|gg ScaI agt|act SfoI ggc|gcc SmaI ccc|ggg SnaBI tac|gta SpeI a|ctagt SphI gcatg|c SspI aat|att SstI gagct|c SstII ccgc|gg StuI agg|cct SwaI attt|aaat TaqI t|cga TliI c|tcgag VspI at|taat XbaI t|ctaga XhoI c|tcgag XmaI c|ccggg sites.

* 每行展示 105 90 75 60 45 30 個堿基

* 爲開(kāi)放(fàng)閱讀框 1 2 3 1, 2, and 3 大(dà)寫 none 展示翻譯信息

* 使用(yòng) standard (1) vertebrate mitochondrial (2) yeast mitochondrial (3) mold mitochondrial (4) invertebrate mitochondrial (5) ciliate nuclear (6) echinoderm mitochondrial (9) euplotid nuclear (10) bacterial (11) alternative yeast nuclear (12) ascidian mitochondrial (13) flatworm mitochondrial (14) Blepharisma macronuclear (15) chlorophycean mitochondrial (16) trematode mitochondrial (21) Scenedesmus obliquus mitochondrial (22) Thraustochytrium mitochondrial (23) 密碼子表.